|
KMID : 1007020090070010015
|
|
Korean Soceity of Osteroporosis
2009 Volume.7 No. 1 p.15 ~ p.21
|
|
|
ALP Activity and Percentage of Viable Cells in C2C12 and MC3T3-E1 Cells according to Alendronate Concentration and Treatment Time
|
|
Jung Min-Hyung
Kim Tak
Kim Heung-Yeol
Jung Hyuk
Lee Tae-Hwa
Eo Wan-Kyu
|
|
|
Abstract
|
|
|
Objectives: To investigate the effect of alendronate on osteoblast proliferation and differentiation using mosue preosteoblast cell line, MC3T3-E1 and myoblastic cell line, C2C12.
Methods: C2C12 and MC3T3-E1 cells were cultured in DMEM media with various concentrations (10-9 M, 10-8 M, 10-7 M, 10-6 M, 10-5 M, 10-4 M) of alendronate for one, two, or three days. After the indicated culture times, MTT assay was performed and alkaline phosphatase activity was examined.
Results: Alendronate had no cytotoxicity regardless of the concentrations used. ALP activity in C2C12 cells was increased in alendronate treated group compared to the control group before 24 hours of treatment, but decreased rapidly after 24 hours of treatment. In MC3T3-E1, it was increased greatly at 10-8 M before 48 hours of treatment and decreased rapidly at more than 10-8 M concentration regardless of times of treatment.
Conclusion: This study shows that alendronate has a dose-dependent effect in osteoblast differentiation. The effect may be associated with the increased expression of ALP.
|
|
|
KEYWORD
|
|
|
Alendronate, Osteoblast differentiation, C2C12 cell, MC3T3-E1 cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|